The PCR reactions was set up by Archana Patel and Emmanuel Boafo-Asare from extracted Orchid DNA samples 4-34 through 4-44. There were no errors in setting up the reaction. The gel electrophoresis process was set up successfully; the only error in prepping was forgetting to add EZ vision to the marker before loading it into the gel. As a result, the marker was not stained and therefore could not be visualized when the gel was imaged.
All the samples were amplified successfully in the PCR with the exception of Adenoncos Sp. in lane B4. This sample failed to amplify either due to having inadequate amount of template to be amplified or failure to add sufficient amount of enzyme to synthesize new templates. Observing the amplified samples, I would estimate the size of the bands to be around 1100Kb even though the marker is not visible to accurately compare the bands.
| Lane Number | DNA Code | Genus Species | PCR Results |
| A1 | 4-34 | Polystachya longiscapa | ++ |
| A2 | 4-35 | Cyrtorchis monteirae | ++ |
| A3 | 4-36 | Tridactyle tridentata | ++ |
| A4 | 4-37 | Jumellea comorensis | ++ |
| A5 | 4-38 | Aerangis biloba | ++ |
| A6 | 4-39 | Phalaenopsis stuartiana | ++ |
| A7 | marker | ||
| A8 | empty | ||
| A9 | empty | ||
| A10 | empty | ||
| B1 | 4-40 | Phalaenopsis amboinensis | ++ |
| B2 | 4-41 | Phalaenopsis gigantea | ++ |
| B3 | 4-42 | Paraphalaenopsis serpentelingua | ++ |
| B4 | 4-43 | Adenoncos sp. | — |
| B5 | 4-44 | Papilionanthe pedunculata | ++ |
| B6 | NC | water | |
| B7 | marker | ||
| B8 | empty | ||
| B9 | empty | ||
| B10 | empty |
ArmstrongGenetics3 
The labeling of the gel image is fine and the table has most of the required components. The table should also include the DNA extraction numbers. In addition, all images should have an appropriate caption (Example: “Figure 1: description of image”.
Title: only “atpF” should be italicized – review assignment to make sure this post has a proper title.
Categorization: This post should only be categorized by the region amplified (under PCR results but do not choose PCR results itself as a category).
Body: Please review the assignment so that you can complete all requirements for this post. Some key components are missing.
Please update post using the above suggestions.
As far as your PCR results are concerned, I think more accurate analysis could be used to determine that high yield only represented 3 of your samples (ie. A-4, A-5, A-6), while your other samples could be argued to represent moderate to low yield. A more accurate analysis will yield more accurate results.