Carly S., Mary C., and Tram N. performed the PCR amplification of DNA’s 4-1 through 4-11 for the targeting region of ycf1. The purpose of this experiment was to amplify using PCR ycf1 from orchid species DNAs 4-1 through 4-11, which were previously extracted. The last lane on the far right in the second row (lane M) served as our marker lane. The products were then collected to run the Agarose Gel Electrophoresis. A total of twelve lanes were loaded as described in figure 1.
Figure 1: Agarose gel electrophoresis of ycf1 gene 4-1 to 4-11. Lane 1: H2O (control). Lane 2-12: ycf1 4-1 to 4-11. Lane M: protein markers
As evident by figure 1, the experiment was sucessful since most of the samples produced either strong (++) or mild (+) success rate, except for 10-y, (lane 11, Cleisostoma subulatum), also shown by table 1. The error could have occurred in several occasions. One sight of possible error was during the loading of the sample into the lanes during the gel electrophoresis. This procedure was obscured when the samples were loaded into the lanes improperly, being loaded while on the bench and not in the proper location. The samples were loaded into the gel wells without being submerged in running buffer in the gel electrophoresis apparatus. However, only one sample was unsuccessful, thus we can assume this error didn’t have an extreme effect on the samples. The relative size of the amplified product was larger than a thousand base pairs.
Table 1: PCR results of ycf1 4-1 to 4-11. The species names were described. (++) denotes a strong amplificaion, (+) denotes an amplification , and (-) denotes a weak ampflication.
This post was an equal collaborative effort of Carly and Tram.
ArmstrongGenetics3 
The gel image is properly labeled. However, there are a number of issues. 4-1 through 4-11 are not primers, they are DNAs from specific orchid species. Primers specific to ycf1 were used to amplify a portion of the sequence from these DNAs. Next, please review your description of the error made in loading your gel. It makes little sense in its current state. Samples should be loaded into gel wells while the gel is submerged in running buffer in a gel electrophoresis apparatus. Other statements also require greater accuracy. For example, the first sample is not a well loaded with water. You had a no DNA (or water) control in your PCR reactions.
This post does not belong under the DNA extractions category (it should only be categorized under ycf1 PCR results). Please correct the categorization of this post.
Missing required information: a table summarizing the results and a description of the relative size of the amplified product (use the marker bands – see Useful Info under Assignments).
Please attempt to make the box around the gel image smaller and include a caption below the gel image stating what it is (this would also be a good place to include “Figure 1: description here”)
Please make the indicated changes to this post. Some other things that need to be rectified:
The post currently appears with two copies of the gel image – this will confuse the reader.
Add “Table 1: __________” above the table when you insert it (see above). The blank should include a brief description of the contents of the Table. When referring to pertinent info in the text then include either (Figure 1), (Table 1) or both.
Can you have a final look at our post. Thank you
These issues have been addressed. Good!
You’ve fixed the double image problem, added informative captions, etc. The species names should be italicized in the table (and any place else they appear) and the post was a “collaborative” effort, not collaborated effort. In the second sentence, the structure needs help. Replace “to perform a PCR amplification on DNA ycf1 4-1 to 4-11” with something like “to amplify using PCR ycf1 from orchid species DNAs 4-1 through 4-11”.
Dr. Jarrell
I don’t know why my post is so weird. When I 1st click on it, there are 2 images of the gel show up, then if I click on the 1st gel, the gel will disappear. I tried to edit it, but in there I didn’t see the image of the 1st gel. I don’t know if you have the same problem when you click on my post or not?
Nevermind. I think I know why now :p. Sorry