Preparation of successfully amplified xdh from DNAs 4-12 to 4-22

Melody Johnson and Kenette Pamphile preformed a cleanup with 7 of the 10 PCR DNA samples.  We were able to “clean up” the reaction by adding exonuclease which removes left over primers and Alkaline PhosphAtase which removes the deoxyribonucleotide triphosphate.  Our results shown in table 1 are from using the nano-drop spectrophotometer.  Our results show a high concentration yield.  The ones not used in this clean up were because they gave little to no visible product.  Those could have resulted from errors during the PCR amplification.

 

Table 1.    Nano-drop spectrophotometer results

Sample		Ng/ul
4-12X		418.23
4-13X		421.62
4-15X		428.06
4-17X		475.45
4-18X		435.19
4-21X		438.29
4-22X	476.08