Preperation of successfully amplified Ycf1 from DNAs 4-1 to 4-11

Table 1: Yield (ng/µL) of products (ycf1 4-1 to 4-11) were collected by using nano-drop technology (2nd column). Date of collecting data was recorded (3rd column).

Mary C., Carly S. and Tram N. performed a PCR cleanup experiment using Nano-drop technology on ten of the original eleven DNA samples, excluding 4-10 (lane 11, refer to PCR post https://armstronggenetics3.wordpress.com/category/results/pcr-data/ycf1/). According to table 1. we obtained a high yield of products, previously evident in our gel (thick bands denote high product concentrations) for the PCR that followed initial DNA extractions. It was necessary to exclude DNA sample 4-10 because of its failure to produce an adequate amount of product, evident by the lack of band in the gel. This may be due to error on our part since, when Carly S. ran the gel she neglected to submerge it in buffer. However it is most likely that the DNA extraction was ineffective and the sample was inadequate to the point that no amplification occurred.

This Post was a collaborative effort of Mary C. and Tram N.

Cyrtorchis monteirae

Cyrtorchis monteirae (Rchb.f) Schltr. is an Angraecoid orchid, further classified in tribe Vandeae and subtribe Aerangidinae. C. monteirae is native to lands between Sierra Leone and Uganda, found as far south as Angola.Cyrtorchis monteirae grows as an epiphyte on forest trees always in close proximity to a water source. It has characteristic long pendent like stems, reaching up tp 80 cm. bearing two rows of dark green flattened stems that taper at both ends giving this plant a rippling appearance. Flowers are widely spaced with a white to creamy green coloration and an orange-tinged spur and flowering occurs between the months of October and November.

Example of flowers: http://www.oakhillgardens.com/htm/orchid_fs_detail.php?prod=2696

The Royal Botanic Gardens, in Kew possess a specimen of Cyrtorchis monteirae,  collected in 1873. The private collector is listed as Monteiro,  oddly enough there seems to be some dispute as to the correct spelling for the Genus species of this orchid. It is occasionally referred to as Cyrtorchis monteiroae.

Extracted Specimen:
Photo taken by Mary Cope from the Private Collection of Dr. David Jarrell at Armstrong Atlantic State University

References:

http://plants.jstor.org/specimen/k000306466

Stewart, Joyce, Johan Hermans, and Bob Campbell. Angraecoid Orchids: Species from the African Region. Portland, Or.: Timber, 2006. Print.