sunbum1988

Melody Johnson and Kenette Pamphile preformed a cleanup with 7 of the 10 PCR DNA samples. We were able to “clean up” the reaction by adding exonuclease which removes left over primers and Alkaline PhosphAtase which removes the deoxyribonucleotide triphosphate. Our results shown in table 1 are from using the nano-drop spectrophotometer. Our results show a high concentration yield. The ones not used in this clean up were because they gave little to no visible product. Those could have resulted from errors during the PCR amplification.

Table 1. Nano-drop spectrophotometer results

Sample		Ng/ul
4-12X		418.23
4-13X		421.62
4-15X		428.06
4-17X		475.45
4-18X		435.19
4-21X		438.29
4-22X	476.08

Sarcoglyphis comberi

Tribe : Vandeae.

Sub tribe : Aeridinae.

Close-up of flowers from extraction specimen. photo taken by Dr. Jarrell

The Sarcoglyphis comberi J.J. Woods is a fairly small orchid. the stem can reach to 7cm long with the leaves showing at 5mm intervals. The Sarcoglyphis is monopodial, which means the stem grows from one bud and the leaves are added each year. The leaves are hard, flat, tough, and dark green. According to J.B Comber the leaves are unequally bi lobed. Which means the part of the leaf that is indented has two lobes. The arrangement of flowers are together reaching 40 cm long at the end of the rainy season in the forest. The flower of this plant reaches 12mm broad and bloom at once. The petals are yellow to light brown in color. The lips of the flower is yellow to violet at the tip. The petals on this plant form a V shape. They are short bloomers and mostly bloom in the fall. They grow best under low light and high moisture. The Sarcoglyphis comberi is found in the G. Halimun complex in west Java at 1,000m in the forest. It is a very rare plant.