After analyzing our PCR products via a DNA gel, we moved forward with 8 of the 9 samples, excluding 4-30 (Diaphananthe pellucida) as we did not detect visible product for this sample (no band was visible on the DNA gel). Our PCR results show high yield for samples: 4-28, 4-29, 4-34, 4-35 and moderate to low yield for samples: 4-31, 4-32, 4-33, 4-36. All products only had bands specific for the petB intron. By using Exonuclease I and Alkaline Phosphotase with incubation at 37°C, we purified our 8 samples of excess primers (single stranded DNA) and dNTPs. Our quantified DNA purification results are illustrated in the following table (Table 1).
Table 1: DNA Quantification via NanoDrop Spectrophotometer
| Sample ID | ng/ul | Date |
| 4-28B | 388.19 | 3/22/2012 |
| 4-29B | 373.6 | 3/22/2012 |
| 4-31B | 404.16 | 3/22/2012 |
| 4-32B | 417.56 | 3/22/2012 |
| 4-33B | 381.19 | 3/22/2012 |
| 4-34B | 369.74 | 3/22/2012 |
| 4-35B | 376.22 | 3/22/2012 |
| 4-36B | 402.79 | 3/22/2012 |
ArmstrongGenetics3 